cryo-em image-processing pipeline Search Results


99
Thermo Fisher cryo em image processing
Cryo Em Image Processing, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cryo em image processing/product/Thermo Fisher
Average 99 stars, based on 1 article reviews
cryo em image processing - by Bioz Stars, 2026-03
99/100 stars
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99
Gatan Inc k3 summit direct electron detector
Flow chart showing the image-processing pipeline for the cryo-EM data of Cdiff EσA/Fdx complexes, starting with <t>6,930</t> <t>dose-fractionated</t> movies collected on a 300-keV Titan Krios (FEI) equipped with a <t>K3</t> Summit direct electron detector (Gatan). Movies were frame-aligned and summed using MotionCor239. CTF estimation for each micrograph was calculated with cryoSPARC240. A representative micrograph is shown following processing by MotionCor239. Particles were auto-picked from each micrograph with cryoSPARC240 Blob Picker and then sorted by 2D classification using cryoSPARC2 to assess quality. The selected classes from the 2D classification are shown. After picking and cleaning by 2D classification, the dataset contained 2,415,902 particles. A subset of particles was used to generate an ab initio templates in cryoSPARC2 and 3D heterogeneous refinement was performed with these templates using cryoSPARC240. One major, high-resolution class emerged, which was polished using RELION41 and further cleaned with two more 3D heterogenous refinements. The final 182,390 particles were refined using cryoSPARC Non-Uniform refinement42.
K3 Summit Direct Electron Detector, supplied by Gatan Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/k3 summit direct electron detector/product/Gatan Inc
Average 99 stars, based on 1 article reviews
k3 summit direct electron detector - by Bioz Stars, 2026-03
99/100 stars
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90
Cell Signaling Technology Inc cryo-em image processing pipeline
Flow chart showing the image-processing pipeline for the cryo-EM data of Cdiff EσA/Fdx complexes, starting with <t>6,930</t> <t>dose-fractionated</t> movies collected on a 300-keV Titan Krios (FEI) equipped with a <t>K3</t> Summit direct electron detector (Gatan). Movies were frame-aligned and summed using MotionCor239. CTF estimation for each micrograph was calculated with cryoSPARC240. A representative micrograph is shown following processing by MotionCor239. Particles were auto-picked from each micrograph with cryoSPARC240 Blob Picker and then sorted by 2D classification using cryoSPARC2 to assess quality. The selected classes from the 2D classification are shown. After picking and cleaning by 2D classification, the dataset contained 2,415,902 particles. A subset of particles was used to generate an ab initio templates in cryoSPARC2 and 3D heterogeneous refinement was performed with these templates using cryoSPARC240. One major, high-resolution class emerged, which was polished using RELION41 and further cleaned with two more 3D heterogenous refinements. The final 182,390 particles were refined using cryoSPARC Non-Uniform refinement42.
Cryo Em Image Processing Pipeline, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cryo-em image processing pipeline/product/Cell Signaling Technology Inc
Average 90 stars, based on 1 article reviews
cryo-em image processing pipeline - by Bioz Stars, 2026-03
90/100 stars
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90
Thermo Fisher titan krios
Flow chart showing the image-processing pipeline for the cryo-EM data of Cdiff EσA/Fdx complexes, starting with 6,930 dose-fractionated movies collected on a <t>300-keV</t> Titan <t>Krios</t> (FEI) equipped with a K3 Summit direct electron detector (Gatan). Movies were frame-aligned and summed using MotionCor239. CTF estimation for each micrograph was calculated with cryoSPARC240. A representative micrograph is shown following processing by MotionCor239. Particles were auto-picked from each micrograph with cryoSPARC240 Blob Picker and then sorted by 2D classification using cryoSPARC2 to assess quality. The selected classes from the 2D classification are shown. After picking and cleaning by 2D classification, the dataset contained 2,415,902 particles. A subset of particles was used to generate an ab initio templates in cryoSPARC2 and 3D heterogeneous refinement was performed with these templates using cryoSPARC240. One major, high-resolution class emerged, which was polished using RELION41 and further cleaned with two more 3D heterogenous refinements. The final 182,390 particles were refined using cryoSPARC Non-Uniform refinement42.
Titan Krios, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/titan krios/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
titan krios - by Bioz Stars, 2026-03
90/100 stars
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90
Thermo Fisher 300 kev
Flow chart showing the image-processing pipeline for the cryo-EM data of Cdiff EσA/Fdx complexes, starting with 6,930 dose-fractionated movies collected on a <t>300-keV</t> Titan <t>Krios</t> (FEI) equipped with a K3 Summit direct electron detector (Gatan). Movies were frame-aligned and summed using MotionCor239. CTF estimation for each micrograph was calculated with cryoSPARC240. A representative micrograph is shown following processing by MotionCor239. Particles were auto-picked from each micrograph with cryoSPARC240 Blob Picker and then sorted by 2D classification using cryoSPARC2 to assess quality. The selected classes from the 2D classification are shown. After picking and cleaning by 2D classification, the dataset contained 2,415,902 particles. A subset of particles was used to generate an ab initio templates in cryoSPARC2 and 3D heterogeneous refinement was performed with these templates using cryoSPARC240. One major, high-resolution class emerged, which was polished using RELION41 and further cleaned with two more 3D heterogenous refinements. The final 182,390 particles were refined using cryoSPARC Non-Uniform refinement42.
300 Kev, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/300 kev/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
300 kev - by Bioz Stars, 2026-03
90/100 stars
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jadas  (JEOL)
90
JEOL jadas
Flow chart showing the image-processing pipeline for the cryo-EM data of Cdiff EσA/Fdx complexes, starting with 6,930 dose-fractionated movies collected on a <t>300-keV</t> Titan <t>Krios</t> (FEI) equipped with a K3 Summit direct electron detector (Gatan). Movies were frame-aligned and summed using MotionCor239. CTF estimation for each micrograph was calculated with cryoSPARC240. A representative micrograph is shown following processing by MotionCor239. Particles were auto-picked from each micrograph with cryoSPARC240 Blob Picker and then sorted by 2D classification using cryoSPARC2 to assess quality. The selected classes from the 2D classification are shown. After picking and cleaning by 2D classification, the dataset contained 2,415,902 particles. A subset of particles was used to generate an ab initio templates in cryoSPARC2 and 3D heterogeneous refinement was performed with these templates using cryoSPARC240. One major, high-resolution class emerged, which was polished using RELION41 and further cleaned with two more 3D heterogenous refinements. The final 182,390 particles were refined using cryoSPARC Non-Uniform refinement42.
Jadas, supplied by JEOL, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/jadas/product/JEOL
Average 90 stars, based on 1 article reviews
jadas - by Bioz Stars, 2026-03
90/100 stars
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96
Gatan Inc k2 summit direct electron detector
Flow chart showing the image-processing pipeline for the cryo-EM data of Cdiff EσA/Fdx complexes, starting with 6,930 dose-fractionated movies collected on a <t>300-keV</t> Titan <t>Krios</t> (FEI) equipped with a K3 Summit direct electron detector (Gatan). Movies were frame-aligned and summed using MotionCor239. CTF estimation for each micrograph was calculated with cryoSPARC240. A representative micrograph is shown following processing by MotionCor239. Particles were auto-picked from each micrograph with cryoSPARC240 Blob Picker and then sorted by 2D classification using cryoSPARC2 to assess quality. The selected classes from the 2D classification are shown. After picking and cleaning by 2D classification, the dataset contained 2,415,902 particles. A subset of particles was used to generate an ab initio templates in cryoSPARC2 and 3D heterogeneous refinement was performed with these templates using cryoSPARC240. One major, high-resolution class emerged, which was polished using RELION41 and further cleaned with two more 3D heterogenous refinements. The final 182,390 particles were refined using cryoSPARC Non-Uniform refinement42.
K2 Summit Direct Electron Detector, supplied by Gatan Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/k2 summit direct electron detector/product/Gatan Inc
Average 96 stars, based on 1 article reviews
k2 summit direct electron detector - by Bioz Stars, 2026-03
96/100 stars
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Image Search Results


Flow chart showing the image-processing pipeline for the cryo-EM data of Cdiff EσA/Fdx complexes, starting with 6,930 dose-fractionated movies collected on a 300-keV Titan Krios (FEI) equipped with a K3 Summit direct electron detector (Gatan). Movies were frame-aligned and summed using MotionCor239. CTF estimation for each micrograph was calculated with cryoSPARC240. A representative micrograph is shown following processing by MotionCor239. Particles were auto-picked from each micrograph with cryoSPARC240 Blob Picker and then sorted by 2D classification using cryoSPARC2 to assess quality. The selected classes from the 2D classification are shown. After picking and cleaning by 2D classification, the dataset contained 2,415,902 particles. A subset of particles was used to generate an ab initio templates in cryoSPARC2 and 3D heterogeneous refinement was performed with these templates using cryoSPARC240. One major, high-resolution class emerged, which was polished using RELION41 and further cleaned with two more 3D heterogenous refinements. The final 182,390 particles were refined using cryoSPARC Non-Uniform refinement42.

Journal: Nature

Article Title: Basis of narrow-spectrum activity of fidaxomicin on Clostridioides difficile

doi: 10.1038/s41586-022-04545-z

Figure Lengend Snippet: Flow chart showing the image-processing pipeline for the cryo-EM data of Cdiff EσA/Fdx complexes, starting with 6,930 dose-fractionated movies collected on a 300-keV Titan Krios (FEI) equipped with a K3 Summit direct electron detector (Gatan). Movies were frame-aligned and summed using MotionCor239. CTF estimation for each micrograph was calculated with cryoSPARC240. A representative micrograph is shown following processing by MotionCor239. Particles were auto-picked from each micrograph with cryoSPARC240 Blob Picker and then sorted by 2D classification using cryoSPARC2 to assess quality. The selected classes from the 2D classification are shown. After picking and cleaning by 2D classification, the dataset contained 2,415,902 particles. A subset of particles was used to generate an ab initio templates in cryoSPARC2 and 3D heterogeneous refinement was performed with these templates using cryoSPARC240. One major, high-resolution class emerged, which was polished using RELION41 and further cleaned with two more 3D heterogenous refinements. The final 182,390 particles were refined using cryoSPARC Non-Uniform refinement42.

Article Snippet: Flow chart showing the image-processing pipeline for the cryo-EM data of Cdiff Eσ A /Fdx complexes, starting with 6,930 dose-fractionated movies collected on a 300-keV Titan Krios (FEI) equipped with a K3 Summit direct electron detector (Gatan).

Techniques: Cryo-EM Sample Prep

Flow chart showing the image-processing pipeline for the cryo-EM data of Cdiff EσA/Fdx complexes, starting with 6,930 dose-fractionated movies collected on a 300-keV Titan Krios (FEI) equipped with a K3 Summit direct electron detector (Gatan). Movies were frame-aligned and summed using MotionCor239. CTF estimation for each micrograph was calculated with cryoSPARC240. A representative micrograph is shown following processing by MotionCor239. Particles were auto-picked from each micrograph with cryoSPARC240 Blob Picker and then sorted by 2D classification using cryoSPARC2 to assess quality. The selected classes from the 2D classification are shown. After picking and cleaning by 2D classification, the dataset contained 2,415,902 particles. A subset of particles was used to generate an ab initio templates in cryoSPARC2 and 3D heterogeneous refinement was performed with these templates using cryoSPARC240. One major, high-resolution class emerged, which was polished using RELION41 and further cleaned with two more 3D heterogenous refinements. The final 182,390 particles were refined using cryoSPARC Non-Uniform refinement42.

Journal: Nature

Article Title: Basis of narrow-spectrum activity of fidaxomicin on Clostridioides difficile

doi: 10.1038/s41586-022-04545-z

Figure Lengend Snippet: Flow chart showing the image-processing pipeline for the cryo-EM data of Cdiff EσA/Fdx complexes, starting with 6,930 dose-fractionated movies collected on a 300-keV Titan Krios (FEI) equipped with a K3 Summit direct electron detector (Gatan). Movies were frame-aligned and summed using MotionCor239. CTF estimation for each micrograph was calculated with cryoSPARC240. A representative micrograph is shown following processing by MotionCor239. Particles were auto-picked from each micrograph with cryoSPARC240 Blob Picker and then sorted by 2D classification using cryoSPARC2 to assess quality. The selected classes from the 2D classification are shown. After picking and cleaning by 2D classification, the dataset contained 2,415,902 particles. A subset of particles was used to generate an ab initio templates in cryoSPARC2 and 3D heterogeneous refinement was performed with these templates using cryoSPARC240. One major, high-resolution class emerged, which was polished using RELION41 and further cleaned with two more 3D heterogenous refinements. The final 182,390 particles were refined using cryoSPARC Non-Uniform refinement42.

Article Snippet: Flow chart showing the image-processing pipeline for the cryo-EM data of Cdiff Eσ A /Fdx complexes, starting with 6,930 dose-fractionated movies collected on a 300-keV Titan Krios (FEI) equipped with a K3 Summit direct electron detector (Gatan).

Techniques: Cryo-EM Sample Prep