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Thermo Fisher
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Gatan Inc
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Cell Signaling Technology Inc
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Thermo Fisher
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Thermo Fisher
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JEOL
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Gatan Inc
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Image Search Results
Journal: Nature
Article Title: Basis of narrow-spectrum activity of fidaxomicin on Clostridioides difficile
doi: 10.1038/s41586-022-04545-z
Figure Lengend Snippet: Flow chart showing the image-processing pipeline for the cryo-EM data of Cdiff EσA/Fdx complexes, starting with 6,930 dose-fractionated movies collected on a 300-keV Titan Krios (FEI) equipped with a K3 Summit direct electron detector (Gatan). Movies were frame-aligned and summed using MotionCor239. CTF estimation for each micrograph was calculated with cryoSPARC240. A representative micrograph is shown following processing by MotionCor239. Particles were auto-picked from each micrograph with cryoSPARC240 Blob Picker and then sorted by 2D classification using cryoSPARC2 to assess quality. The selected classes from the 2D classification are shown. After picking and cleaning by 2D classification, the dataset contained 2,415,902 particles. A subset of particles was used to generate an ab initio templates in cryoSPARC2 and 3D heterogeneous refinement was performed with these templates using cryoSPARC240. One major, high-resolution class emerged, which was polished using RELION41 and further cleaned with two more 3D heterogenous refinements. The final 182,390 particles were refined using cryoSPARC Non-Uniform refinement42.
Article Snippet: Flow chart showing the image-processing pipeline for the cryo-EM data of Cdiff Eσ A /Fdx complexes, starting with 6,930 dose-fractionated movies collected on a 300-keV Titan Krios (FEI) equipped with a
Techniques: Cryo-EM Sample Prep
Journal: Nature
Article Title: Basis of narrow-spectrum activity of fidaxomicin on Clostridioides difficile
doi: 10.1038/s41586-022-04545-z
Figure Lengend Snippet: Flow chart showing the image-processing pipeline for the cryo-EM data of Cdiff EσA/Fdx complexes, starting with 6,930 dose-fractionated movies collected on a 300-keV Titan Krios (FEI) equipped with a K3 Summit direct electron detector (Gatan). Movies were frame-aligned and summed using MotionCor239. CTF estimation for each micrograph was calculated with cryoSPARC240. A representative micrograph is shown following processing by MotionCor239. Particles were auto-picked from each micrograph with cryoSPARC240 Blob Picker and then sorted by 2D classification using cryoSPARC2 to assess quality. The selected classes from the 2D classification are shown. After picking and cleaning by 2D classification, the dataset contained 2,415,902 particles. A subset of particles was used to generate an ab initio templates in cryoSPARC2 and 3D heterogeneous refinement was performed with these templates using cryoSPARC240. One major, high-resolution class emerged, which was polished using RELION41 and further cleaned with two more 3D heterogenous refinements. The final 182,390 particles were refined using cryoSPARC Non-Uniform refinement42.
Article Snippet: Flow chart showing the image-processing pipeline for the cryo-EM data of Cdiff Eσ A /Fdx complexes, starting with 6,930 dose-fractionated movies collected on a
Techniques: Cryo-EM Sample Prep